A Minimal Optical Trapping and Imaging Microscopy System

A Minimal Optical Trapping and Imaging Microscopy System

We report the construction and testing of a simple and versatile optical trapping apparatus, suitable for visualizing individual microtubules (∼25 nm in diameter) and performing single-molecule studies, using a minimal set of components. This design is based on a conventional, inverted microscope, operating under plain bright field illumination. A single laser beam enables standard optical trap...

Macro-optical trapping for sample confinement in light sheet microscopy.

Light sheet microscopy is a powerful approach to construct three-dimensional images of large specimens with minimal photo-damage and photo-bleaching. To date, the specimens are usually mounted in agents such as agarose, potentially restricting the development of live samples, and also highly mobile specimens need to be anaesthetized before imaging. To overcome these problems, here we demonstrat...

Determination of experimental Tumor Volume of a mouse using optical imaging system in different processing stages

Cryoimaging-Microscopy Implementation for 3D Optical Imaging

Coherent nonlinear optical imaging: beyond fluorescence microscopy.

The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems. Coherent nonlinear optical imaging, especially the recently developed nonlinear dissipation microscopy (including stimulated Raman scattering and two-photon absorption) and pump-probe microscopy (including excited-sta...